Error: not a Phred33 file


I am trying to import some data for analysis. I keep coming up on this error saying that my csv file is not a Phred33. I know for sure it’s not a Phred64, but I tried that anyway. Unsurprisingly, this did not work. I am running qiime2 through vitural box (window). Here is my manifest file.sequences.csv (295 Bytes)

Any suggestions?image1%20(5)

Thank you for your support!

(Matthew Ryan Dillon) #2

(Matthew Ryan Dillon) #3

Can you please upgrade to the latest version of QIIME 2 (2018.11), we are unable to support a version that is over a year old. Also, the latest version contains improvements to this error message that will be more informative for you. Thanks! :t_rex: :qiime2: