Hi all,
I am new to the forum. I am working through the tutorial Training feature classifiers with q2-feature-classifier Training feature classifiers with q2-feature-classifier — QIIME 2 2020.2.0 documentation.
Unfortunately I am running into issues because I am not using the most up-to-date version of qiime2. I managed to solve the first issue at the qiime tools import part by reverting back to the old code
--source-format HeaderlessTSVTaxonomyFormat \ which is now......
--input-format HeaderlessTSVTaxonomyFormat
However, I cannot update my version of qiime2 because I am working remotely and accessing our HPC via a vpn. I don't have the permission to update.
I am now running into an issue at the stage of Extract reference reads part of the tutorial....
I run the following code,
but receive this error
Error: no such option: --p-min-length
I have tried to set --p-min-length to 0 so this removed this but I still run into issues.
This is the code I ran to get the above error meassge
qiime feature-classifier extract-reads
--i-sequences 85_otus.qza
--p-f-primer GTGCCAGCMGCCGCGGTAA
--p-r-primer GGACTACHVGGGTWTCTAAT
--p-trunc-len 120
--p-min-length 100
--p-max-length 400
--o-reads ref-seqs.qza
I just wondered if anyone had experienced this issue. I couldn't find anything when I searched this time.
Thanks, Kate