Hello,
I got q2-picrust2 plug in installed and then ran the command mentioned below:
qiime picrust2 full-pipeline
--i-table table-dada2.qza
--i-seq rep-seqs-dada2.qza
--output-dir q2-picrust2_output
--p-placement-tool sepp
--p-threads 1
--p-hsp-method pic
--p-max-nsti 2
--verbose
For this, I am getting this error message Warning - 143 input sequences aligned poorly to reference sequences (--min_align option specified a minimum proportion of 0.8 aligning to reference sequences). These input sequences will not be placed and will be excluded from downstream steps.
Is there some thing wrong with my input files?