Hi,
It looks like there is an issue with the linked reference set file in the most recent version (at least 2024.5) of the Parkinson's Mouse Tutorial: https://data.qiime2.org/2024.5/common/sepp-refs-gg-13-8.qza. Wget gives a 404 error and does not work at all, and trying to upload using curl/direct file transfer seems to work, but then when you run the next step in the workflow (qiime fragment-insertion), it gives an error saying that the reference file is not a QIIME archive. I am able to run this step successfully using older versions of the reference file (i.e., 2023.5).