Hi everyone,
I am trying to perform picrust2 analysis. The tree had been successfully constructed. The next step, however, has resulted in the following error:
qiime picrust2 custom-tree-pipeline --i-table '/media/sf_compartida/Proyecto/Analisis_primer_seg/Filtrado/birds_primer_seg_table_filtered-ffff.qza' --i-tree '/media/sf_compartida/Proyecto/PICRUST/primer_analis_fragment_pricust--verbose/tree.qza' --output-dir '/media/sf_compartida/Proyecto/PICRUST/q2-picrust2-output-primer' --p-threads 1 --p-hsp-method pic --p-max-nsti 2 --verbose
Running the below commands:
hsp.py -i 16S -t /tmp/tmp31dw214p/placed_seqs.tre -p 1 -n -o /tmp/tmp31dw214p/picrust2_out/16S_predicted.tsv.gz -m pic -e 0.5
Error running this command:
hsp.py -i 16S -t /tmp/tmp31dw214p/placed_seqs.tre -p 1 -n -o /tmp/tmp31dw214p/picrust2_out/16S_predicted.tsv.gz -m pic -e 0.5
Standard error of the above failed command:
Traceback (most recent call last):
File "/home/qiime2/miniconda/envs/qiime2-2021.11/bin/hsp.py", line 4, in
import('pkg_resources').run_script('PICRUSt2==2.4.1', 'hsp.py')
File "/home/qiime2/miniconda/envs/qiime2-2021.11/lib/python3.8/site-packages/pkg_resources/init.py", line 656, in run_script
self.require(requires)[0].run_script(script_name, ns)
File "/home/qiime2/miniconda/envs/qiime2-2021.11/lib/python3.8/site-packages/pkg_resources/init.py", line 1453, in run_script
exec(code, namespace, namespace)
File "/home/qiime2/miniconda/envs/qiime2-2021.11/lib/python3.8/site-packages/PICRUSt2-2.4.1-py3.8.egg/EGG-INFO/scripts/hsp.py", line 145, in
main()
File "/home/qiime2/miniconda/envs/qiime2-2021.11/lib/python3.8/site-packages/PICRUSt2-2.4.1-py3.8.egg/EGG-INFO/scripts/hsp.py", line 121, in main
check_files_exist([args.tree, trait_table])
File "/home/qiime2/miniconda/envs/qiime2-2021.11/lib/python3.8/site-packages/PICRUSt2-2.4.1-py3.8.egg/picrust2/util.py", line 406, in check_files_exist
raise ValueError("This input file was not found: " + missing_files[0])
ValueError: This input file was not found: /home/qiime2/miniconda/envs/qiime2-2021.11/lib/python3.8/site-packages/PICRUSt2-2.4.1-py3.8.egg/picrust2/default_files/prokaryotic/16S.txt.gz
Could you kindly assist me?
Thanks in advance.