I am using the same primer pair 515F/806R. I think that I will then just continue on with forward reads, I did a testrun with few samples:
qiime dada2 denoise-single --i-demultiplexed-seqs import-single-end.qza --p-trunc-len 122 --o-representative-sequences dada2-rep-seqs.qza --o-table dada2-table.qza --o-denoising-stats dada2-stats.qza
and this time it worked and came back without any errors. However I am not entirely sure how to determine where to truncate it exactly. Here I did it at 122 is that okay or what is the best way to decide that? I also read about a method called FIGARO that can help with that. Do you have any knowledge about usage of FIGARO and would recommend using it? https://www.biorxiv.org/content/10.1101/610394v1
