Error in ANCOM plugin

[fellora (Felipe Huanachin Carahuanco)]

Hi Everyone,

I’m working in qiime2-2021.11.0 and I have this error message when i ran this:

qiime composition ancom
--i-table ANCOM_ready_table.qza
--m-metadata-file categorias_boiling_ok.tsv
--m-metadata-column Paper_Station
--o-visualization ancom_group_results.qzv
--verbose

I have this error message

Plugin error from emperor:

There were some problems with the command:
(1/2) Missing option '--m-metadata-column'.
(2/2) Got unexpected extra argument (Type)

I collapsed to the genus level. I upload the qza files in case it works for you. Thank you very much in advance.

ANCOM_ready_table.qza (130.2 KB)
categorias_boiling_ok.tsv (1.8 KB)

[lizgehret]

Hi @fellora,

Thanks for reaching out!

I ran your exact commands with the files you provided, and was not able to reproduce this error (ancom ran successfully). But based on your error message, I suspect there may have been some sort of typo when you ran your commands. Try copy/pasting the command below, making sure you are located in the same directory as your input files:

qiime composition ancom \
--i-table ANCOM_ready_table.qza \
--m-metadata-file categorias_boiling_ok.tsv \
--m-metadata-column Paper_Station \
--o-visualization ancom_group_results.qzv \
--verbose

This should work as expected, but please let us know if you are still running into issues!

Cheers