I am very new to bioinformatics and am analyzing a dietary dataset. I’m running QIIME 2 version 2020.11 through WSL and Ubuntu. I have run into some trouble while attempting to import my data. I ran into an error while trying to import my dating using the Casava 1.8 format, so decided to try the manifest format with 2 of my samples after following the manifest import tutorial.
Code I ran to import:
qiime tools import \
Error that I’m getting:
There was a problem importing pe-33-manifest.tsv:
pe-33-manifest.tsv is not a(n) PairedEndFastqManifestPhred33V2 file:
Filepath on line 1 and column “forward-absolute-filepath” could not be found ($PWD/pe-33/842026339_S44_L001_R1_001.fastq.gz) for sample “842026339”.
Here are the 2 samples I am attempting to import:
My manifest.tsv file:
Any help is much appreciated, thank you!