/software/software/QIIME2/2018.2/lib/python3.5/site-packages/matplotlib/init.py:962: UserWarning: Duplicate key in file "/home/p277936/.config/matplotlib/matplotlibrc", line #17
(fname, cnt))
There was a problem importing manifest.txt:
/local/1498484/q2-SingleLanePerSampleSingleEndFastqDirFmt-_1v7m77v/CH3E_50_L001_R1_001.fastq.gz is not a(n) FastqGzFormat file:
Missing sequence for record beginning on line 5
Manifest file: NOTE: they dont even are named as in the error appears
Let's break down the error message: in the file for the forwards reads of sample CH3E, the fastq record beginning on line 5 is missing sequence data.
Is there screenshot you provided for sample CH3E? If not, can you send along a screenshot for that sample?
They look right to me - you specified the sample ID is CH3E, and that was is what is reflected in the filename during import (CH3E_50_L001_R1_001.fastq.gz). The first part of the filename is the sample ID. The rest is described here: Importing data — QIIME 2 2018.6.0 documentation
The underscore-separated fields in this file name are the sample identifier, the barcode sequence or a barcode identifier, the lane number, the read number, and the set number.
Basically, everything besides the sample ID and the read direction don't really serve a purpose at this stage (and on) in an analysis.
Hope that helps, looking forward to your reply! :qiime2:
