Error feature-classifier extract-reads

[Nicholas_Bokulich]

Hi @DKioroglou,
Please see this topic for a solution:

A problem to train a classifier for paired-end data from MiSeq!

Dear ALL, I used Qiime2 to have done alpha diversity and beta diversity analysis of my customer data. I’m in the step to do Taxonomic analysis by following the moving -pictures in tutorials. I had a problem to train a classifier for paired-end reads. The sequence length is 300bps. The 16S Amplicon PCR Forward primer F=TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG and the Reverse primer R= GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC. I used the following commands using Greengene…

Good luck!