I am re-analyzing a dataset that I’ve previously, and successfully, analyzed using a 2018 version of qiime2, this time using versions 2019.4, and 2019.10. Here is my command:
qiime demux emp-paired
This time, I keep getting the following error:
ValueError: No sequences were mapped to samples. Check that your barcodes are in the correct orientation (see the rev_comp_barcodes and/or rev_comp_mapping_barcodes options).
These are golay barcodes that should be in the forward primer with no need to do the reverse complement. Does anyone have any idea of why this isn’t working this time? Are there any changes that occurred between 2018 and 2019 that I am missing?