Editing metadata in a .qza file

I have demultiplexed, quality controlled, aligned sequences as single .fastq and single .fna files for each sample. I want to use QIIME2 for diversity and taxonomic analysis only.

Importing the .fna files with the following command

qiime tools import --type ‘SampleData[Sequences]’ --output-path outfile.qza --input-path manifest.csv --source-format SingleEndFastqManifestPhred33

I have an associated metadata.yml file. However, I see no way to incorporate it into data that is already demultiplexed. If I open the .qza file with an editor [TextWarngler], I see a metadata.yaml as follows:

uuid: 9fb71406-2bd4-404a-a11d-8ef73805f8e6
type: SampleData[SequencesWithQuality]
format: SingleLanePerSampleSingleEndFastqDirFmt

I want to replace this with my actual metadata.yaml file. TextWrangler won’t let me edit this. What should I do?

Hi there @mshelomi!

Please see this quote from @Mehrbod_Estaki, from your last topic on this forum:

Specifically, the link to the metadata guide at the bottom. In general though I would recommend you take a step back and review the documentation and tutorials we have put together at docs.qiime2.org — the fundamental ideas behind QIIME 2 are described in great detail there, and will hopefully steer you in the right direction, generally speaking. As always, we are here to help!

Thanks! :t_rex: :qiime2:

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