Duplicate sample IDs (with underscores) in Casava format

[thermokarst]

Hi @charisssep!

@charisssep

I have checked my samples again and can not find any duplicates.

Can you provide some additional details, so that we can assist you:

1. What version of QIIME 2 are you running?
2. What were the exact commands you ran - you mentioned above that you tried both deblur and dada2, please include both if possible
3. What are the exact errors you are seeing? Please attach the log files if possible, otherwise copy-and-paste the error messages.
4. Can you please provide a brief sample of your sample IDs? One or two should be plenty.

@charisssep

I have tried running both Dada2 and Deblur and am also encountering a Duplicate Sample IDs error for both.

It sounds like deblur doesn't like underscores, but this shouldn't be the case in dada2 (to the best of my recollection). Your answers to the above questions will help guide our analysis of the issue you reported above.

@charisssep

Do you have a recommendation for what step prior to either Dada2 or Deblur that I should fix my samples?

Before commenting on this we will need to see your answers to the questions (1-4) above.

Thanks!!