Duplicate of KOs in function prediction with picrust2 or tax4fun

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Dear all,
I am confused by the results of function prediction with picrust2 or tax4fun. Here is my question. For example, the Ko0001 involved in different metabolism processes.
K00001 Carbohydrate metabolism
K00001 Lipid metabolism
K00001 Amino acid metabolism
K00001 Metabolism of cofactors and vitamins
K00001 Xenobiotics biodegradation and metabolism
How the softwares ( picrust2 or tax4fun) handle these duplicated information for each 16s rRNA gene ? The same value (abundance or relative abundance) was assigned to different functions?

Could anyone do me a favor and explain this question?

Best wishes

Long

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Hi @LXE_Soil_ecol

Since the same KO identifier has been used for multiple pathways, I would assume it is present in each and therefore is a contributor for every pathway listed. It must therefore play a role in all of these various metabolic pathways.

I would imagine if it only listed unique KO identifiers, then you would miss vital information regarding the multifunctional nature of many of these orthologs in your microbial communities.

I hope that helps!