Duplicate Barcodes

I have 16S metagenomics data of many samples in multiple runs of MiSeq. I want to analyze my data using qiime. The problem is its giving the error that the barcode sequence is duplicate. Since in multiple runs I have used same barcodes thats why its giving this error. Can anybody suggest me something.

Hi @Junaid!

Since you have multiple runs, you’ll want to create a SampleData[SequencesWithQuality] artifact for each run (meaning you import each run separately and won’t see the barcode issue), then you can analyze each with dada2 and merge the tables afterwards.

We have a tutorial on that workflow which you should check out!

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