Does QIIME pipeline is adapted to analyse the results of sequencing of ITS and 16SrDNA at once ?

General Discussion
1

Dear all,

I’m asking if QIIME2 is adapted to analyse the results of sequencing of the ITS and the 9 hypervariables regions of 16SrDNA at once.

If no, do you have an idea about a pipeline used to analyse the aforementioned sequencing data ?

Thank you for your help

2

Hello Meriem,

One easy option would be to use the vsearch plugin to run cluster-features-closed-reference, but there are some big limitations to closed-ref clustering and you may have better options.

Can you tell me more about the primers used to create these amplicons? Are these similar to the Ion Torrent metagenomics kit discussed here?

Colin

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