Does disabling error correction lead to a loss of reads?

[thermokarst]

Hey there @wlandesman!

In short, yes. Demultiplexing using an exact match approach. Check out this post I recently wrote on the topic.

emp demultiplexing: No sequences were mapped to samples

Hi @Yavnika! The safest way is to check with your sequencing center! Check with your sequencing center to be sure. Error correction helps you recover more sequences per sample by correcting any read errors in the barcode. With correction disabled you will lose any sequences where the barcode has an error in it (an A called as a C, for example). If you don't have many errors in your barcodes, then error correction might not be necessary. If you're comfortable with the percentage of your reads…

I hope that helps!