does ancom test need more than 2 replicates?

Thank you so much
It make sense
Sorry for alot of questions, i added this question now

Hi @arar,

Its easier if you ask the additional question as a new entry or a new post.

But, yes, ANCOM is a test that wants more than two replicates. Assume that pretty much every statistical test you want to run requires more than two replicates if for no other reason than because of power.



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