Difficulty with Greengenes2 2022.10

Okay. The full length Naive Bayes classifier given the primers. You may be able to run the Docker container of QIIME 2 via Singularity which is not uncommon in HPC environments. An alternative to closed reference with non-v4-16s is to place with SEPP ( Arbitrary ASV fragment placement in Greengenes2 )

Best,

Daniel