differential abundance bar plot for the specified category:

[hardik007 (hardik thobhanbhai)]

I am doing qiime2 analysis for my samples and i got this below error while i am visualizing of my da-barplot-Sample72filt-Type.qzv file on qiime2view:

Error : * Couldn't generate plot for Samplesupernatant: No features remaining after applying filters.

this is the command i have used : qiime composition ancombc
--i-table "/home/apinnaka/ambika_lot_final/table-no-mito-no-chl.qza"
--m-metadata-file "/home/apinnaka/ambika_lot_final/metadata72new.txt"
--p-formula 'Sample'
--p-reference-levels 'Sample::Cell'
--o-differentials ancombc-Sample-Type.qza
Saved FeatureData[DifferentialAbundance] to: ancombc-Sample-Type.qza
(qiime2) [apinnaka@genomics ambika_72newclssifier]$ qiime composition da-barplot
--i-data ancombc-Sample-Type.qza
--p-significance-threshold 0.05
--o-visualization da-barplot-Sample72filt-Type.qzv
Saved Visualization to: da-barplot-Sample72filt-Type.qzv

here is the metadata file which is i am using and i want to analyse differential abundance im bacterial community between Cell and supernatant group

[colinvwood (Colin Wood)]

Hello @hardik007,

I believe what happened is that there were no features found to be differentially abundant between the "Sample" groups below a significance threshold of 0.05.

[hardik007 (hardik thobhanbhai)]

Thanks for your response.