Different taxa result from DADA2 and Deblur

[benjjneb (Ben Callahan)]

@Setiawan

I have another question, why the sequence improving very much? Previously, I set my trunc-len based on quality of forward and reverse reads, so I set my --p-trunc-len-r 205 (demux.qzv) and got very low sequences.

Because when you set --p-trunc-len-f 250 --p-trunc-len-r 205 your reads didn't overlap, hence essentially all wer were lost in merging, but but now they do. Schematic...

FFFFFFF(250)FFFFFFFF
                      RRRRR(205)RRRRR

FFFFFFF(250)FFFFFFFF
                 RRRRRR(250)RRRRRRRRR