Different annotations for seqs and its corresponding reverse seqs

[Nicholas_Bokulich]

Hi @111,
Your sequence data are in mixed orientations.

As documented a few times on this forum, the classify-sklearn method specifically (but not other taxonomy classifiers) assumes that the data are in a single orientation.

Thus, you must orient your reads prior to classifying. Fortunately, we have a new plugin to achieve that, see the tutorial here:

Processing, filtering, and evaluating the SILVA database (and other reference sequence data) with RESCRIPt

Please consider this tutorial a living document, which may change based upon community feedback and ongoing plugin development. RESCRIPt RESCRIPt (REference Sequence annotation and CuRatIon Pipeline) is a python package and QIIME 2 plugin for formatting, managing, and manipulating sequence reference databases. This package was designed for compiling, manipulating, and evaluating sequence reference databases from SILVA, NCBI, Greengenes, GTDB, and other sources, and for construc…

Or use one of the other taxonomy classification methods in q2-feature-classifier.

Good luck!