dada2 denoise-single to get the ASVs: rep-seqs_qza.
And then I annotated the seqs with the downloaded
qiime feature-classifier classify-sklearn \ --i-classifier silva-132-99-nb-classifier.qza --i-reads rep-seqs.qza
In my results, I found two seqs are the same, seqA and seqB.
seqB is the reverse complement of seqA.
However, I found the annotations reports which generated by
qiime feature-classifier classify-sklearn
are complete different for them.
So, is there something wrong?