Hi,
I am doing denoise-paired using following command qiime dada2 denoise-paired --i-demultiplexed-seqs demux-paired-end.qza --p-trim-left-f 10 --p-trim-left-r 10 --p-trunc-len-f 240 --p-trunc-len-r 240 --o-table table.qza --o-representative-sequences rep-seqs.qza --o-denoising-stats denoising-stats.qza --p-n-threads 0 --verbose
and its showing error, please see the attached image. I have 32 core, 512 GB memory but
when i am using less thread like 10, its taking more than 30 hrs and its still running (see another image).
Hello @Khem. The more threads you create, the more memory you end up using. I suspect that when using all 32 cores you are running out of memory and that is manifesting as the error you are seeing. Depending on the size of your data it isn’t unusual for you to run out of memory or for a run on ten cores to be taking a very long time.
Additionally, what version of QIIME 2 are you running, and what kind of environment is this? If it is a virtual machine, is the core count and memory amount quoted the amount for the system as a whole or the amount available to the virtual machine?