denoise-paired error

[Nicholas_Bokulich]

Hi @idupanloup,
This is a pretty common technical issue that has been discussed many times on the forum: Search results for 'Calls: removeBimeraDenovo -> isBimeraDenovoTable order:latest' - QIIME 2 Forum

E.g., see here for an explanation:

dada2 denoise-paired error using qiime 2019.4

Hi @danielsebas! Yes indeed, right this way: This error happens when your DADA2 is unable to join any of your reads. It is possible that your inclusion of so many low-quality nt positions is causing this. Any chance you can tighten up your trim/trunc params? This will depend on the length of the target region in question. Another option is to also proceed with only the forward reads. :qiime2:

Good luck!