Hi @danielsebas!
Yes indeed, right this way:
This error happens when your DADA2 is unable to join any of your reads. It is possible that your inclusion of so many low-quality nt positions is causing this. Any chance you can tighten up your trim/trunc params? This will depend on the length of the target region in question. Another option is to also proceed with only the forward reads.
:qiime2: