Hii,
I was running the code to summarize the De-multiplexed sequences. code was as below--
qiime demux summarize --i-data demux_seqs.qza --o-visualization visualization.qzv
But i am getting the following error-
Plugin error from demux:
'NoneType' object has no attribute 'strip'
Debug info has been saved to /tmp/qiime2-q2cli-err-kan1eryz.log
Looking forward to suggestions
Hi @Shan,
Please re-run your command with the --verbose flag and copy/paste the results in your response - this will give us a better idea of where this error is coming from. Thanks!
Its giving the error like-
Traceback (most recent call last):
File "/home/shabbir/anaconda3/envs/qiime2-2023.5/lib/python3.8/site-packages/q2cli/commands.py", line 468, in call
results = action(**arguments)
File "", line 2, in summarize
File "/home/shabbir/anaconda3/envs/qiime2-2023.5/lib/python3.8/site-packages/qiime2/sdk/action.py", line 274, in bound_callable
outputs = self.callable_executor(
File "/home/shabbir/anaconda3/envs/qiime2-2023.5/lib/python3.8/site-packages/qiime2/sdk/action.py", line 558, in callable_executor
ret_val = self._callable(output_dir=temp_dir, **view_args)
File "/home/shabbir/anaconda3/envs/qiime2-2023.5/lib/python3.8/site-packages/q2_demux/_summarize/_visualizer.py", line 140, in summarize
for seq in _read_fastq_seqs(filename):
File "/home/shabbir/anaconda3/envs/qiime2-2023.5/lib/python3.8/site-packages/q2_demux/_demux.py", line 64, in _read_fastq_seqs
yield (seq_header.strip(), seq.strip(), qual_header.strip(),
AttributeError: 'NoneType' object has no attribute 'strip'
Hi @Shan,
Thanks for providing that traceback!
It looks like the issue lies somewhere within one of your sequence or quality score headers. Let's take a closer look at your demux file - can you run the following command and copy/paste the results in your response?
qiime tools validate demux_seqs.qza
This should give us more information on where the issue lies within your demultiplexed sequences. Thanks! 
Hello @Shan,
It looks like you deleted your last post. We won't plan on responding until you update this topic again, just to let you know.
thanks, colin. the problem is resolved
This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.