Demux summarize on already demultiplexed data

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1

I have paired-end data that is already demultiplexed and imported using the generic fastq commands. However, to move on to any quality filtering I have to be able to view the summary of the demultiplexing results, am I correct?

This is the command I run:

qiime demux summarize \
  --i-data paired-end-demux.qza \
  --o-visualization demux.qzv

When I run the summary table command, I receive the error:

Plugin error from demux:

  Invalid DISPLAY variable

Debug info has been saved to /tmp/qiime2-q2cli-err-zoh9ypke.log.

Is there a suggested method for data that is paired-end and already demultiplexed to move on to the quality filtering step?

Let me know if I need to provide more information.

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3

Hi @morganpuff! This error isn't related to your data or qiime demux summarize, it's a system configuration issue with your matplotlib installation. matplotlib's default configuration requires a display, which you don't appear to have (this is common if you're logged into a server and don't have an X server and X-forwarding set up).

This topic will show you how to configure matplotlib to work in a headless environment. Get in touch if you have any issues!

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5

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