I'm super new to QIIME2 and I got the same error messages when conducting 'demux summarize' several times.
I think I've gotten the sequencing data which have been already demultiplexed ('sequences are separated into different files based on the kind of sample.'), so I jumped into the 'demux summarize' step to see the quality scores after importing data.
Here is the code I used, the error messages, and the log I got.
qiime demux summarize --i-data paired-end-demux.qza --o-visualization demux.qzv
Plugin error from demux:
CRC check failed 0xe020340 != 0xac99c05f
Debug info has been saved to /tmp/qiime2-q2cli-err-f9s_ppuj.log
Traceback (most recent call last):
File "/home/ryunhee/anaconda3/envs/qiime2-2022.11/lib/python3.8/site-packages/q2cli/commands.py", line 352, in call
results = action(**arguments)
File "", line 2, in summarize
File "/home/ryunhee/anaconda3/envs/qiime2-2022.11/lib/python3.8/site-packages/qiime2/sdk/action.py", line 234, in bound_callable
outputs = self.callable_executor(scope, callable_args,
File "/home/ryunhee/anaconda3/envs/qiime2-2022.11/lib/python3.8/site-packages/qiime2/sdk/action.py", line 443, in callable_executor
ret_val = self._callable(output_dir=temp_dir, **view_args)
File "/home/ryunhee/anaconda3/envs/qiime2-2022.11/lib/python3.8/site-packages/q2_demux/_summarize/_visualizer.py", line 140, in summarize
for seq in _read_fastq_seqs(filename):
Followings are troubleshootings I tried after googling;
- Re-download fastq files (.fq.gz) from the sequencing center
- Re-import data (.fq.gz --> .qza)
BUT It didn't work at all ...
I would be really grateful if you provide any suggestions for me.
Thanks in advance..!
P.S. There were no problems at all at the importing data step.
Here are codes and some parts of manifest file used in the 'importing data' step;
qiime tools import
Sample-id/ forward-absolute-filepath/ reverse-absolute-filepath (column separated)
Sample-1/ $PWD/microbiome-data/CM1B_1.fq.gz/ $PWD/microbiome-data/CM1B_2.fq.gz