demultiplexing?

as in the tsv just maps barcodes to samples but you do not have a separate barcode sequence file?

choose your own adventure:

Where are your barcodes?

  1. in-line with the sequences
    a. see the q2-cutadapt tutorial
  2. in the header line or some place else
    a. :sob: QIIME 2 does not have a way to handle this right now (or maybe ever? this is an unusual format) :sob:
    b. figure out a way to extract barcodes from the headers into a separate file (qiime1 had a way to do this I think)
    c. import and process as EMP.