Demultiplexing with 4 Fastq files R1 , R2, I1, I2and how toconstruct mapping file with dual barcodes

Hi @Mehrbod_Estaki,

Thank you. But, In my case, I have single trim.contigs.qual file and fasta files are only present for each sample. I donot have qual file for each sample. So, what would be the approach only with fasta files per sample. How to import only the fasta files in qiime2 for downstream analysis.

I see there is a post for the similar situation as the one I have, How to import cleaned multi-sample reads?. Will this approach help.

Thanking you,
Sravani.