I am new to QIIME2, I have read and practise most of the QIIME2 tutorials and tried my best to solve my problem.
I got the demultiplex data with barcode and primer trimmed, the data was from Hiseq PE 250, but I failed to import them into QIIME2. I am not sure about the the meaning of the format, like in my mac:
qiime tools import --show-importable-formats
I check for the importable format but some formats are not mentioned in the tutorial, that’s why I am not sure about how I can import my data. May I know anywhere I can check for the detail of the formats?
My data has R1 file and R2 file for one sample, the forward sequence looks like:
HIIIHHIIIIIIIIIIIIIIIGIHHHIIIIIIIHIIHIIIIIIIIIIIIIIIIHIIIIHIIIHFHIIGHHHIIHHIIFFEHIIGIIIIIIIIIGHHIHGIIHIDGHFHIIIHHI[email protected][email protected]@CEH>DHFHGI-@EH45CH—[email protected]@C@@-6-@F#######
I tried the most possible format and follow the instruction in the tutorial in my mac:
qiime tools import
but the error is:
There was a problem importing test:
Missing one or more files for CasavaOneEightSingleLanePerSampleDirFmt: ‘.+_.+_L[0-9][0-9][0-9]_R_001\.fastq\.gz’
Any help and suggestion is grateful!