Demuliplex-sequences still contan primers, how can you run DADA2?

[thermokarst]

Hi @Cheng50373640!

@Cheng50373640

How can you run DADA2 after import demuliplexed sequences?

The q2-dada2 methods actually only accept demultiplexed sequences as input, so this is perfect!

@Cheng50373640

because they still contain primers or/and barcodes.

You can use the q2-cutadapt plugin to remove primers or barcodes. Check out this community tutorial for examples.

Hope that helps! :qiime2: