Hallo!
I have a problem:
I have used degenerate primers, I need to use the script “qiime feature-classifier extract-reads”
but it doesn’t work. No errors but almost empty and very strange output.
qiime feature-classifier extract-reads
–i-sequences classifier.qza
–p-f-primer TGCGCTAGGTCAAATTGAAA
–p-r-primer GTTTCWGGGTTACCRAACATYACACC
–o-reads classifier_extract.qza
What do you suggest me?
Thank you very much,
Lisa