I have a question about the columns in the deblur stats artefact generated by the
--o-stats flag when running
qiime deblur denoise-16S.
A few of my samples have very low numbers of input reads (likely a failed PCR or poor quality input). When I look at the deblur stats they are
0 in every column after
reads-raw. I assume this is because there are no non-singleton reads in my samples so everything is removed in the first step. Is this correct? If not, can somebody please explain what is happening.
I have included the relevant samples from the output and command used below for reference (the input
demux-filtered.qza is the output from
qiime quality-filter q-score).
All the best,
qiime deblur denoise-16S \ --i-demultiplexed-seqs demux-filtered.qza \ --p-trim-length 150 \ --p-sample-stats \ --p-jobs-to-start 8 \ --p-no-hashed-feature-ids \ --o-table feature-table.qza \ --o-representative-sequences rep-seqs.qza \ --o-stats deblur-stats.qza