Deblur Plugin Set-up

Hi @Taylorr,

I have some good news and some bad news... with a heavy caveat.

So, the bad news first:
QIIME 2 will not support applying deblur to your data because it no longer has quality filtering. I'm not entirely sure if deblur will work as a base algorithm (although its within the qiime2 enviroment). Typically, dereplicated sequences don't go into denoising, since quality information is an important part of the process.
There are absolutely ways to move forward with this data, as long as you also have the table that associates counts of dereplicated sequences to your samples; I'm happy to talk you through this if its the best route.

Your other option (if you cna manage it) would be to reach out to the person who provided the data and see if they have fastq files for you. Your sequencing provider might have access to them, or the person who did dereplication for you. This would be my recommendation if you can get access to the data. I think having raw data makes it easier to know what you did and how that affects your results.

Best,
Justine

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