Hi Jai,
Below is the return on verbose.
(qiime2-2017.10) dyn-160-39-172-19:Qiime_Good Harry$ qiime deblur denoise-16S --i-demultiplexed-seqs gi-filtered.qza **--p-trim-length -1** --o-representative-sequences rep-seqs-deblur2.qza --o-table table-deblur2-1.qza --o-stats deblur2-stats.qza --verbose
Traceback (most recent call last):
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/bin/deblur", line 4, in <module>
__import__('pkg_resources').run_script('deblur==1.0.3', 'deblur')
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/pkg_resources/__init__.py", line 748, in run_script
self.require(requires)[0].run_script(script_name, ns)
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/pkg_resources/__init__.py", line 1517, in run_script
exec(code, namespace, namespace)
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/deblur-1.0.3-py3.5.egg-info/scripts/deblur", line 684, in <module>
deblur_cmds()
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/click/core.py", line 722, in __call__
return self.main(*args, **kwargs)
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/click/core.py", line 697, in main
rv = self.invoke(ctx)
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/click/core.py", line 1066, in invoke
return _process_result(sub_ctx.command.invoke(sub_ctx))
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/click/core.py", line 895, in invoke
return ctx.invoke(self.callback, **ctx.params)
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/click/core.py", line 535, in invoke
return callback(*args, **kwargs)
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/deblur-1.0.3-py3.5.egg-info/scripts/deblur", line 632, in workflow
threads_per_sample=threads_per_sample)
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/deblur/workflow.py", line 879, in launch_workflow
error_dist, indel_prob, indel_max)
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/deblur/deblurring.py", line 114, in deblur
seqs = get_sequences(input_seqs)
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/deblur/deblurring.py", line 65, in get_sequences
", ".join(map(str, unaligned_lengths))))
ValueError: **Not all sequence have the same length.** Aligned lengths: 630, sequence lengths: 386, 315, 319, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 366, 368, 376
Traceback (most recent call last):
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/q2cli/commands.py", line 218, in __call__
results = action(**arguments)
File "<decorator-gen-328>", line 2, in denoise_16S
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/qiime2/sdk/action.py", line 220, in bound_callable
output_types, provenance)
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/qiime2/sdk/action.py", line 355, in _callable_executor_
output_views = self._callable(**view_args)
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/q2_deblur/_denoise.py", line 96, in denoise_16S
hashed_feature_ids=hashed_feature_ids)
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/site-packages/q2_deblur/_denoise.py", line 163, in _denoise_helper
subprocess.run(cmd, check=True)
File "/Users/Harry/miniconda3/envs/qiime2-2017.10/lib/python3.5/subprocess.py", line 398, in run
output=stdout, stderr=stderr)
subprocess.CalledProcessError: Command '['deblur', 'workflow', '--seqs-fp', '/var/folders/lz/dshtc4351ns3418pq2yq5wph0000gn/T/qiime2-archive-kq94fo7s/e8827318-b6d8-49b5-8ed2-a48574d85c25/data', '--output-dir', '/var/folders/lz/dshtc4351ns3418pq2yq5wph0000gn/T/tmpdz2g9rzq', '--mean-error', '0.005', '--indel-prob', '0.01', '--indel-max', '3', '--trim-length', '-1', '--min-reads', '10', '--min-size', '2', '--jobs-to-start', '1', '-w']' returned non-zero exit status 1
Plugin error from deblur:
Command '['deblur', 'workflow', '--seqs-fp', '/var/folders/lz/dshtc4351ns3418pq2yq5wph0000gn/T/qiime2-archive-kq94fo7s/e8827318-b6d8-49b5-8ed2-a48574d85c25/data', '--output-dir', '/var/folders/lz/dshtc4351ns3418pq2yq5wph0000gn/T/tmpdz2g9rzq', '--mean-error', '0.005', '--indel-prob', '0.01', '--indel-max', '3', '--trim-length', '-1', '--min-reads', '10', '--min-size', '2', '--jobs-to-start', '1', '-w']' returned non-zero exit status 1
See above for debug info.
probably that is the issue that the length of the sequences are not the same.