Hello,
I’m running the commands (sown at the end) and realized that in the visualization outputs of
- qiime metadata tabulate
- qiime deblur visualize-stats
contain the full feature-ids which I used to load the reads;
However the
- qiime deblur visualize-stats
output visualization contains only truncated feature ids; I have used the “–p-no-hashed-feature-ids” parameter, but this does not solve the problem.
01_U341F-U806R-NW3-1
10_U341F-U806R-NW3-11
11_U341F-U806R-NW3-12
(…)
get truncated to
01
10
11
(…)
qiime deblur denoise-16S
–i-demultiplexed-seqs 2a_Biogas3b-all_deblur-filtered.qza
–p-trim-length 272
–o-representative-sequences 2b_Biogas3b-all_deblur-rep-seqs.qza
–o-table 2b_Biogas3b-all_deblur-table.qza
–p-sample-stats
–p-jobs-to-start 12
–o-stats 2b_Biogas3b-all_deblur-stats.qza
qiime metadata tabulate
–m-input-file 2a_Biogas3b-all_deblur-filter-stats.qza
–o-visualization 2c_Biogas3b-all_deblur-filter-stats.qzv # full feature ids present
qiime deblur visualize-stats
–i-deblur-stats 2b_Biogas3b-all_deblur-stats.qza
–o-visualization 2d_Biogas3b-all_deblur-stats.qzv # full feature ids present
qiime feature-table summarize
–i-table 2b_Biogas3b-all_deblur-table.qza
–o-visualization 2e_Biogas3b-all_deblur-joined-table.qzv
–m-sample-metadata-file metadata-Biogas3b.txt
This command gives me an error (The following IDs are not present in the metadata: ‘01’, ‘02’, ‘03’, ‘04’, ‘05’, ‘06’, ‘07’, ‘08’, ‘09’, ‘10’, ‘11’, ‘12’, ‘13’, ‘14’, ‘15’), which is true since the metadate file contains the full feature ids.
If I run it without “–m-sample-metadata-file metadata-Biogas3b.txt”, the command completes but the visualization has now the truncated feature ids.
So, I assume that the previous artifacts di still have the full feature ids.
Does anybody have an idea what could cause the shortening of the feature ids?
Best regards
