I am using deblur through QIIME2-2022.8 for 16S data (v3-v4 regions) and I saw many posts about this error message from deblur but, as far I could see, not related to NovaSeq data.:
No sequences passed the filter. It is possible the trim_length (25) may exceed the longest sequence, that all of the sequences are artifacts like PhiX or adapter, or that the positive reference used is not representative of the data being denoised.
Plus, the data quality seems okay, as you can see here:
demux.qzv (306.9 KB)
Here you go my commands (actually, I did many trials setting wider gaps for trimmings):
qiime deblur denoise-16S --i-demultiplexed-seqs demux-filtered.qza --p-trim-length 25 --p-left-trim-len 128 --o-representative-sequences rep-seqs.qza --o-table table.qza --p-sample-stats --o-stats deblur-stats.qza
Could you provide me some info that I'm probably missing?
Thank you so much for your reply in advance!