data import error

I am new to qiime2.

  1. I am getting a following error when importing my data.
    There was a problem importing MANIFESTSheet1.tsv:

MANIFESTSheet1.tsv is not a(n) PairedEndFastqManifestPhred33V2 file:

'forward-absolute-filepath' is not a column in the metadata. Available columns: 'absolute-filepath''

  1. I have 3 samples AC, NC and BS (each with R1 and R2 reads) with no barcode sequences. my sample reads are named as follows
  2. Is there a issue in my manifest file. manifest file is in tsv format.
    sample-id absolute-filepath
    AC1 /root/Data/reads/AC1_R1.fq.gz
    AC2 /root/Data/reads/AC2_R2.fq.gz
    BS1 /root/Data/reads/BS1_R1.fq.gz
    BS2 /root/Data/reads/BS2_R2.fq.gz
    NC1 /root/Data/reads/NC1_R1.fq.gz
    NC2 /root/Data/reads/NC2_R2.fq.gz
  3. import command used are
    qiime tools import
    --type 'SampleData[PairedEndSequencesWithQuality]'
    --input-path MANIFESTSheet1.tsv
    --output-path paired-end-demux.qza
    --input-format PairedEndFastqManifestPhred33V2

An off-topic reply has been merged into an existing topic: error occured whemn importing data

Please keep replies on-topic in the future.

Hi @priti,

As the error suggest, the format of the manifest file you using is not what qiime2 expects for a ManifestPhred33V2. Your manifest it is more similar the old ManifestPhred33.

Both manifests are discussed in the following thread, which may help you in solve your problem:

Hope it helps.

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