I have fastq.gz files of 12 samples (100 bp per read), approximately 33 GB total. Dataset are generated using WGS.
I can successfully execute dmux.qza and demux.qzv.
qiime dada2 denoise-paired **
–i-demultiplexed-seqs demux.qza **
–p-trim-left-f 0 **
–p-trim-left-r 0 **
–p-trunc-len-f 99 **
–p-trunc-len-r 89 **
–o-table table.qza **
–o-representative-sequences rep-seqs.qza **
While executing, it failed to run, after 12 hours it shows plugin error.
My computer RAM:32 GB, HD-500GB, I
What RAM size is needed?