DADA2 truncation parameter and negative control filtering

Hello!

There was this big argument discussion about negative controls and what to do about contamination. What do you think about all of this?


They are supposed to have no reads, but clearly they do, so we need to understand where these reads are coming from to choose our next steps.

Based on peoples ideas from that last thread and other threads, what do you think you should do? What do you think is the best way to handle the real reads that should not be in a negative control?

Colin

1 Like