Hi, I am having the following error when I try to denoise and trim my qiime imported demultiplexed paired-end dataset. <a name="p-7301-command-qiime-dada2-denoise-paired-i-demultiplexed-seqs-demux-casava_paired-endqza-o-table-table-o-representative-sequences-rep-seqs-p-trim-left-f-22-p-trim-left-r-18-p-trunc-len-f-225-p-trunc-len-r-218-1" class="anchor" href="#p-7301-command-qiime-dada2-denoise-paired-i-demultiplexed-seqs-demux-casava_paired-endqza-o-table-table-o-representative-sequences-rep-seqs-p-trim-left-f-22-p-trim-left-r-18-p-trunc-len-f-225-p-trunc-len-r-218-1" aria-label="Heading link"> Command: qiime dada2 denoise-paired --i-demultiplexed-seqs demux-casava_paired-end.qza --o-table table --o-representative-sequences rep-seqs --p-trim-left-f 22 --p-trim-left-r 18…

Hi @parachrosis ! Sorry to hear things aren't going well :frowning:. Unfortunately, we see this dada2 -9 exit code error happen when a machine runs out of memory. The easiest solution is to run this somewhere with more memory --- if you are on an academic cluster you could request more RAM. If not, y…

DADA2 trimming and denoising issue for demultiplexed paired-end amplicons from MiSeq