Dada2 trim/trunc

Quick question regarding sequence trimming/truncation with dada2. I’m trying to understand --p-trim-left-f/r and --p-trunc-len-f/r. Do these specify relative or absolute positions, and which order are they applied in? Since --p-trim-left-f/r has the potential to change the length of the sequences, it affects what value I would specify for --p-trunc-len-f/r if it specifies relative values.

Hi @ngarcia,
These would be absolute positions so the ordering doesn’t matter.

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