I do have query regarding dada2 output file rep-seqs.qza:
16s V4 region was amplified by using the PCR primers (F515/R806), and sequencing read length for both pair end read is 250bp.
dada2 rep-seqs.qzv file gives min. seq. length is 229 and max is 436. I am not able to understand it when fragment length between forward and reverse primer is 291, how it can be 436