DADA2 out of memory error

Hello Fred,

Yep, I mean reducing the training data set using --p-n-reads-learn.

To speed this process up, you might consider renting a super-computer from Amazon and running qiime on it. This process is a little technical the first time you set it up, but can remove a major bottleneck in your analysis.

Seriously, have you considered renting a super-computer? I've highlighted three good ones on this list, and they cost under $2 a hour. For the price of running PCR on one sample, you could do all this analysis and get some results. Amazon EC2 Instance Comparison

Colin

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