dada2 or vsearch

[llenzi (Luca)]

Hi @yogurt
With deblur you get a kind of ASVs, please look at:

To cluster or not to cluster?

Hey all, I'm always intrigued by this topic so I just thought I'd throw in my 2 cents as well, if nothing at least I'll have somewhere to reference in future posts. But I look forward to reading other views on the matter. First just to clarify terminology because sometimes using the wrong terms can add extra confusion, especially to newcomers. The product of denoising methods such as DADA2, DEBLUR, UNOISE, (and perhaps MED?) are Exact Sequence Variants (ESV). These different methods all arrive…

In which Mehrod brilliantly discussed all about ASVs and clustering!

For the Ns, there are options to filter out input sequences with errors, please see '--p-max-ee-f'
and '--p-max-ee-f' in denoise-paired: Denoise and dereplicate paired-end sequences — QIIME 2 2020.8.0 documentation

Hope it helps