Richard
#1
like this, and this is the code i run
qiime dada2 denoise-paired \
--i-demultiplexed-seqs paired-end-demux.qza \
--p-trim-left-f 0 \
--p-trim-left-r 0 \
--p-trunc-len-f 250 \
--p-trunc-len-r 250 \
--o-table table.qza \
--o-representative-sequences rep-seqs.qza \
--o-denoising-stats denoising-stats.qza \
--p-n-threads 0
and the QC result:
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Richard
#2
sequence has a okay result with FLASH and PEAR
and this is the result ,this first one is the sample i failed
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Richard
#3
and when i did join-read, the same question occurred.i don't know why there are such error,but the
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Good to hear from you, Rick,
Looks like reads are not pairing consistently…
Try triming that forward read a little sooner, say 240 or 230. That should still give you 40 or 30 bp of overlap.
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