A different question related to mock-20 data set run.
We run different settings to check if result is optimal. One thing we notice that in DADA2 run by QIIME2, non-chimera removal step has huge difference with one parameter minFoldParentOverAbundance = 1.0. Default using this parameter, we got 13291 reads are non-chimera. But if not using this parameter, we got 59097 reads are non-chimera. Do you know why it has huge differences?
Sorry to bother you so many times.