Hi everyone. Nice to be back with a new problem 
. I have seen around forum with this error code but couldn't find the one similar to mine.
I am currently running Qiime2-2020.2 and analyzing 1012 x single end fastq files.I have used the "manifest file import SingleEndFastqManifestPhred33V2" protocol to import my fastq run files in .qza artifacts for analysis. I have successfully ran the dada2 denoise-single command on single-end-demux.qza yesterday. And was also able to produce table.qza and rep-seq.qza successfully.
But I made a mistake with Filenames so I planed to re-analyse the data with correct filenames.I have successfully produced single-end demux.qza file but ran into problem during data2 denoise part.I ran into the following error:
Plugin error from dada2:
** An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.**
Log file:
cat /tmp/qiime2-q2cli-err-nv2jv9mz.log
**R version 3.5.1 (2018-07-02) **
Loading required package: Rcpp
**DADA2: 1.10.0 / Rcpp: 1.0.3 / RcppParallel: 4.4.4 **
1) Filtering ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................
2) Learning Error Rates
192186935 total bases in 1023509 reads from 26 samples will be used for learning the error rates.
3) Denoise samples .................................................................................Error in dada_uniques(names(derep[[i]]$uniques), unname(derep[[i]]$uniques), : **
** Bad lambda.
Calls: dada -> dada_uniques
Execution halted
Running external command line application(s). This may print messages to stdout and/or stderr.
The command(s) being run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist.Command: run_dada_single.R /tmp/qiime2-archive-2kfi64qt/a5818789-cbbc-4415-bc34-98495d69e528/data /tmp/tmp5_l50h9j/output.tsv.biom /tmp/tmp5_l50h9j/track.tsv /tmp/tmp5_l50h9j 0 0 2.0 2 Inf consensus 1.0 1 1000000 NULL 16
Traceback (most recent call last):
** File "/home/qamar/miniconda3/envs/qiime2-2020.2/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 177, in _denoise_single**
** run_commands([cmd])**
** File "/home/qamar/miniconda3/envs/qiime2-2020.2/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 36, in run_commands**
** subprocess.run(cmd, check=True)**
** File "/home/qamar/miniconda3/envs/qiime2-2020.2/lib/python3.6/subprocess.py", line 418, in run**
** output=stdout, stderr=stderr)**
subprocess.CalledProcessError: Command '['run_dada_single.R', '/tmp/qiime2-archive-2kfi64qt/a5818789-cbbc-4415-bc34-98495d69e528/data', '/tmp/tmp5_l50h9j/output.tsv.biom', '/tmp/tmp5_l50h9j/track.tsv', '/tmp/tmp5_l50h9j', '0', '0', '2.0', '2', 'Inf', 'consensus', '1.0', '1', '1000000', 'NULL', '16']' returned non-zero exit status 1.
My conditions are --p-trim-left 0 \ --p-trunc-len 0 \ for dada2 and using them with "nohup". I am feeling there is something wrong going on with tmp folder by looking at the log file. I will be glad to receive input from you guys. Thanks in advance.